Centre for Proteome Research

Welcome to the LIV-SRF Centre for Proteome Research

The LIV-SRF proteomics facility works alongside the highly successful Centre for Proteome Research.  The facility operates 10 mass spectrometry platforms as well as the necessary on and offline separation technologies for proteins and peptides.  These support a wide range of proteomic analyses. We have extensive experience in a range of analysis methodologies including quantification, phosphorylation analysis, imaging and much, much more.

In addition to the world-class technology housed in the Centre for Proteome Research, we offer full consultancy and support from experimental planning to data analysis. We take pride in our work and want you to get the best quality data using the most appropriate techniques. If you are not sure what you want or how to do it, get in touch and we will be happy to discuss your requirements with you.

We run a Proteins for the Masses day to showcase our facility once a year, this gives you the opportunity to find out more about us and have an informal chat about your project.

Capabilities

Our wide range of state-of-the-art instrumentation enables us to analyse both routine and unusual samples. We are able to cater for most types of samples and studies.

What we do:


Protein Discovery

The bread and butter of our service perfect for identifying large numbers of proteins or peptides.

Quantitative Proteomics

There are many different types of quantification methodologies. We are capable of doing both absolute quantification (QconCAT methods) and relative quantification (SILAC and TMT methods).

  • Protein Characterisation
  • Protein characterisation encompasses several methodologies:
  • De novo protein sequencing
  • Protein Identification
  • Localisation of post-translational modifications
  • Intact protein analysis

We also have a selection of ionisation methods that widen our capabilities.

  • Rapid Evaporative Ionisation Mass Spectrometry (REIMS)
    • Ionises unprocessed samples, used for creating spectral patterns and classifying sample type
  • Matrix-assisted laser desorption ionisation
    • Used for quick analysis of simple mixtures or small non-volatile molecules
      Also capable of imaging tissue sections and includes a matrix sprayer and digestion station
  • Desorption electrospray ionisation
    • Used for the imaging of tissue sections
  • Gas-Chromatography Mass Spectrometry
    • Used for the analysis of small volatile molecules
  • Ion-mobility
    • Used for detecting conformational effects of ligand binding

Instruments

We have 10 instruments of 7 varieties:

  • Thermo Q-Exactive
  • Thermo Q-Exactive HF
  • Thermo Fusion
  • Thermo Lumos
  • Waters Xevo TQ
  • Waters Synapt G2Si HDMS
  • Waters GCT Premier

Each instrument is capable of coupling to at least one of the online separation techniques or specialist ionisation sources listed below:

  • Thermo U3000 UPLC
  • Waters NanoAquity UPLC
  • SCIEX 8000+ capillary electrophoresis
  • Thermo HESI Probe
  • Waters REIMS Source
  • Waters MALDI Source
  • Waters DESI Source
  • Thermo Nano-Spray source


Other offline capabilities include:

  • High pH Fractionation
  • Protein purification
  • De-Salting
  • Phospho-enrichment

For more information please visit our website.

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