Tick and insect cell lines

As well as tick cell lines established at the University of Edinburgh between 1980 and 2012, at The Pirbright Institute between 2012 and 2017 and at the University of Liverpool since 2017, the Tick Cell Biobank holds many cell lines deposited by Professors Ulrike Munderloh and Timothy Kurtti of the University of Minnesota and Dr Patricia Holman of Texas A&M University.

In addition, we hold two of the original Rhipicephalus appendiculatus cell lines (RA243 and RA257) established at the London School of Hygiene and Tropical Medicine over 40 years ago by Varma, Pudney and Leake (1975), and two deposits of the RML-15 cell line set up at Rocky Mountain Laboratories, Montana, USA in the late 1970s by Yunker, Cory and Meibos (1985). RML-15 was published as originating from Dermacentor variabilis embryos, but both of our deposits were found to be derived from Rhipicephalus sanguineus; as the exact origin of these cells is unclear and we do not know if they were derived from the same or different primary cultures, for convenience we refer to the low passage deposit as “RML-15” and the high passage deposit as “RML-RSE”. Recent deposits include two new cell lines derived from Brazilian Rhipicephalus microplus (RBME-6, Lima-Duarte et al., 2021) and Amblyomma sculptum (ASE-14, Lima-Duarte et al., 2022).

The table below details the tick and insect cell lines currently available for distribution.

Please email the Tick Cell Biobank for further information on these and other tick cell lines not listed in the table.

Other arthropod cell lines

We currently hold cell lines derived from two midge, two sand fly, two triatomid bug, one honey bee and four mosquito species available for distribution; these are also listed in the table below.

Back to: Faculty of Health and Life Sciences

 

Arthropod group

Species

Origin

Line

Instar

Approx. passage

Incubation temperature

Reference

Mosquito Aedes aegypti India Aag2 Larva Unknown 28°C Singh, 1967; Lan and Fallon, 1990
Mosquito Aedes albopictus India U4.4 Larva Unknown 28°C Singh, 1967; Miller and Brown, 1992

Mosquito

Aedes albopictus

India

C6/36

Larva

Unknown

28°C

Singh, 1967; Igarashi, 1978

Hard tick

Amblyomma americanum

United States

AAE2

Embryo

28

32°C - 34°C

Kurtti et al., 2005

Hard tick

Amblyomma americanum

United States

AAE12

Embryo

8

32°C - 34°C

Singu et al., 2006

Hard tick

Amblyomma sculptum

Brazil

ASE-14 Embryo 

 15

28°C - 30°C Lima-Duarte et al., 2022 

Hard tick

Amblyomma variegatum

Southern Africa

AVL/CTVM13

Moulting larva

150

28 - 32°C, 37°C

Bell-Sakyi et al., 2000

Hard tick

Amblyomma variegatum

Southern Africa

AVL/CTVM17

Moulting larva

123

32°C

Bell-Sakyi, 2004

Honey Bee

Apis melifera

United States

AME-711

Embryo

35

32°C

Goblirsch et al., 2013

Soft tick

Argas reflexus

Germany

ARE/LULS41

Embryo

14

28°C

Bell-Sakyi et al., 2018

Soft tick

Carios capensis

United States

CCE1

Embryo

132

32°C - 34°C

Mattila et al., 2007

Soft tick

Carios capensis

United States

CCE2

Embryo

112

32°C - 34°C

Mattila et al., 2007

Mosquito

Culex pipiens complex

United Kingdom

CPE/LULS50

Embryo

40

28°C

Bell-Sakyi et al. (2021)

Mosquito

Culex pipiens molestus

United Kingdom

CPE/LULS56

Larva

20

28°C

Bell-Sakyi et al. (2021)

Mosquito

Culex quinquefasciatus

 

Hsu

Adult

Unknown

28°C

Hsu et al., 1970

Biting midge

Culicoides nubeculosus

United Kingdom

CNE/LULS44

Embryo

16

28°C

Bell-Sakyi et al., 2020

Biting midge

Culicoides nubeculosus

United Kingdom

CNE/LULS47

Embryo

10

28°C

Bell-Sakyi et al., 2020

Biting midge

Culicoides sonorensis

United States

KC

Embryo

62

28°C

Wechsler et al., 1991

Hard tick

Dermacentor (Anocentor) nitens

United States

ANE58

Embryo

15

32°C - 34°C

Kurtti and Munderloh, 1983

Hard tick

Dermacentor albipictus

United States

DALBE3

Embryo

15

32°C - 34°C

Policastro et al., 1997

Hard tick

Dermacentor andersoni

United States

DAE15

Embryo

55

32°C - 34°C

Simser et al., 2001

Hard tick

Dermacentor andersoni

United States

DAE100T

Embryo

60

32°C - 34°C

Kurtti et al., 2005

Hard tick

Dermacentor reticulatus

Germany

DRE/LULS60

Embryo

8

28°C

Bell-Sakyi et al., 2022

Hard tick

Dermacentor variabilis

United States

DVE1

Embryo

10

32°C - 34°C

Kurtti et al., 2005

Hard tick

Hyalomma anatolicum

India

HAE/CTVM8

Embryo

163

32°C

Bell-Sakyi, 1991

Hard tick

Hyalomma anatolicum

India

HAE/CTVM9

Embryo

212

32°C

Bell-Sakyi, 1991

Hard tick

Hyalomma dromedarii

East Africa

HDE/PIPA33

Embryo

20

28°C

Bell-Sakyi et al., 2018

Hard tick

Hyalomma dromedarii

East Africa

HDE/LURF39

Embryo

12

28°C

Bell-Sakyi et al., 2018

Hard tick

Hyalomma lusitanicum

Spain

HLE/LULS42

Embryo

8

28°C

Bell-Sakyi et al., 2018

Hard tick

Hyalomma lusitanicum

Spain

HLE/LULS43

Embryo

15

28°C

Bell-Sakyi et al., 2022

Hard tick

Hyalomma lusitanicum

Spain

HLE/LULS48

Embryo

15

28°C

Bell-Sakyi et al., 2022

Hard tick

Ixodes ricinus

Spain

IRE/LUAP46

Embryo

8

28°C

Bell-Sakyi et al., 2022

Hard tick

Ixodes ricinus

Spain

IRE/LULS55

Embryo

20

28°C

Bell-Sakyi et al., 2022

Hard tick

Ixodes ricinus

United Kingdom

IRE/CTVM19

Embryo

230

28°C

Bell-Sakyi et al., 2007

Hard tick

Ixodes ricinus

United Kingdom

IRE/CTVM20

Embryo

180

28°C

Bell-Sakyi et al., 2007

Hard tick

Ixodes ricinus

Germany

IRE11

Embryo

56

32°C - 34°C

Simser et al., 2002

Hard tick

Ixodes scapularis

United States

IDE12

Embryo

15

28°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

IDE2

Embryo

86

32°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

IDE8

Embryo

102

32°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

ISE6

Embryo

83

32°C - 34°C

Kurtti et al., 1996

Hard tick

Ixodes scapularis

United States

ISE18

Embryo

55

32°C - 34°C

Munderloh et al., 1994

Sand fly

Lutzomyia longipalpis

Brazil

LLE/LULS45

Embryo

25

28°C

Bell-Sakyi et al., 2021

Sand fly

Lutzomyia longipalpis

Brazil

LLL/LULS52

Larva

20

28°C

Bell-Sakyi et al., 2021

Sand fly

Lutzomyia longipalpis

Brazil

LLE/LULS40

Embryo

50

28°C

Bell-Sakyi et al., 2018

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM21

Embryo/neonate larva

60

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM22

Embryo/neonate larva

60

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM24

Embryo/neonate larva

59

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM27

Embryo

47

28°C

Bell-Sakyi et al., 2009

Sand fly

Phlebotomus papatasi

Turkey

PPL/LULS49

Larva

20

28°C

Bell-Sakyi et al., 2021

Hard tick Rhipicephalus appendiculatus Kenya RAE/CTVM1 Embryo 95 32°C Bell-Sakyi, 2004
Hard tick Rhipicephalus appendiculatus Kenya RAN/CTVM3 Moulting nymph 70 28°C Bekker et al., 2002

Hard tick

Rhipicephalus appendiculatus Kenya RAE/PIPM38 Embryo

7

28°C Bell-Sakyi et al., 2018
Hard tick Rhipicephalus appendiculatus Kenya RAE25 Embryo 143 28°C - 34°C Kurtti et al., 1982
Hard tick Rhipicephalus appendiculatus East Africa RA243 Moulting nymph 358 28°C - 32°C Varma et al., 1975

Hart tick

Rhipicephalus appendiculatus

East Africa

RA257

Moulting nymph

264

28°C - 32°C

Varma et al., 1975

Hard tick

Rhipicephalus (Boophilus) decoloratus

Kenya

BDE/CTVM14

Embryo

100

28°C

Lallinger et al., 2010

Hard tick

Rhipicephalus (Boophilus) decoloratus

Kenya

BDE/CTVM16

Embryo

106

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Costa Rica

BME/CTVM2

Embryo

160

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Costa Rica

BME/CTVM4

Embryo

150

28°C

Bell-Sakyi et al., 2007

Hard tick

Rhipicephalus (Boophilus) microplus

Colombia

BME/CTVM5

Embryo

90

28°C

Bell-Sakyi et al., 2007

Hard tick

Rhipicephalus (Boophilus) microplus

Colombia

BME/CTVM6

Embryo

250

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Mozambique

BME/CTVM23

Embryo

77

32°C

Alberdi et al., 2012

Hard tick

Rhipicephalus (Boophilus) microplus

Mozambique

BME/CTVM30

Embryo

45

28°C

Alberdi et al., 2012 

Hard tick

Rhipicephalus (Boophilus) microplus

Mexico

BmVIII-SCC

Embryo

65

32°C

Holman, 1981

Hard tick

Rhipicephalus (Boophilus) microplus

Brazil

BME/PIBB36

Embryo

10

28°C

Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus (Boophilus) microplus

Mexico

BME26

Embryo

88

32°C

Kurtti et al., 1988

Hard tick

Rhipicephalus (Boophilus) microplus

Brazil

RBME-6  Embryo  10  28°C - 30°C  Lima-Duarte et al., 2021 

Hard tick

Rhipicephalus bursa

Spain

RBE/LULS58

Embryo

8

28°C

Bell-Sakyi et al., 2022

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM28

Embryo

53

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM29

Embryo

8

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM31

Embryo

32

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REN/PIPA34

Moulting nymph

20

28°C

Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus sanguineus

United States

RSE8

Embryo

34

32°C - 34°C

Kurtti and Munderloh, 1982

Hard tick

Rhipicephalus sanguineus

United States

RML-RSE

Embryo

100

28°C - 32°C

Yunker et al. 1981; Bell-Sakyi et al., 2015

Hard tick

Rhipicephalus sanguineus

United States

RML-15

Embryo

20

28°C

Yunker et al. 1981; Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus sanguineus

France

RSE/PILS35

Embryo

20

28°C

Koh-Tan et al., 2016

Triatomid bug

Rhodnius prolixus

 Venezuela

RPE/LULS53

Embryo

19

28°C

Penrice-Randal et al., 2022

Triatomid bug

Rhodnius prolixus

Venezuela

RPE/LULS57

Embryo

13

28°C

Penrice-Randal et al., 2022

Triatomid bug

Triatoma infestans

Paraguay

TIE/LULS54

Embryo

12

28°C

Penrice-Randal et al., 2022