Next Generation Sequencing

Next Generation Sequencing

At the CGR we provide access to all major sequencing and array platforms, offering a wide range of applications, with advice, informatics and analytical processing of data. The CGR’s status as a reference site often facilitates access to the latest technology prior to general release.

NGS technologies still represent a substantial investment to individual labs and require a great deal of technical and bioinformatic expertise. As a dedicated Centre offering the major short and long-read platforms, the CGR enables researchers to access the very latest technologies as well as established platforms. We facilitate best practices to ensure individual clients receive the maximum return on their investment. CGR offers services which can combine the use of several platforms including Illumina NovaSeq 6000, PacBio Sequel, and 10X Genomics Chromium, to match the needs of researchers. Please consult our team for the best sequencing strategy to achieve the most comprehensive view of your genome of interest. The CGR is certified service provider for Illumina and PacBio platforms, as well as the NimbleGen and Agilent Sure Select sequence capture methods.


llumina

At the CGR, we have 6 Illumina next generation sequencers:  Three MiSeq personal sequencers, one NovaSeq 6000, one HiSeq 2500 and one HiSeq 4000.

 

 The NovaSeq 6000 is the latest sequencing platform launched by Illumina which will provide users with the throughput, speed, and flexibility to complete projects quicker and more economically than ever before. Applications requiring large amounts of data, such as human whole-genome sequencing (WGS), ultradeep exome sequencing, and tumor–normal profiling can now be completed in a more cost-effective manner.

 

 

The MiSeq is an integrated bench top sequencer. It features significantly faster cycle times allowing small projects to be completed quickly at a lower cost. The‌ MiSeq delivers high precision variant detection and is suitable for small-scale projects such as amplicon or small genome sequencing. ‌

 

The HiSeq instruments enable the largest and most complex sequencing studies at the lowest cost. The ability to process larger numbers of samples and to decode larger and more complex genomes means that virtually any sequencing project is now within reach. The HiSeq 2500 has two run modes; high-throughput and rapid while the HiSeq 4000 offers higher capacity for larger genomes and differential expression studies.

 
APPLICATIONS:
  • Resequencing and De novo Sequencing and Assembly of Microbial, Human, Plants and Animal Genomes
  • Targeted Sequencing to produce in-depth views of genetic variation for phylogenetic studies (16S rRNA, 18S rRNA, ITS etc)
  • Whole Transcriptome Analysis to gain a comprehensive view of total RNA and non-coding RNA (RNA-Seq, Small RNA-Seq)
  • Whole Exome or Targeted Sequencing to analyse protein-coding regions of the genome
  • Epigenetics to understand how base modification can affect gene regulation in microbial and eukaryotic organisms
  • Metagenomics enables microbiologists to evaluate bacterial, archaea and eukaryotic diversity and detect the abundance of microbes in various environments

 

PacBio                            

 

The Sequel System provides higher throughput, more scalability and lower sequencing costs than the PacBio RS II System. This allows users to tailor their experimental design and budget, whilst obtaining the benefits of SMRT technology.

       

       

 

 

APPLICATIONS:
  • De novo Sequencing and Assembly of Microbial, Human, Plant and Animal Genomes
  • Targeted Sequencing to produce in-depth views of genetic variation for BAC sequencing and full-length target genes (e.g. HLA alleles)
  • Iso-Seq for sequencing of full-length RNA transcripts and detection of splice variants
  • Epigenetics to achieve a complete view of microbial and eukaryotic DNA modifications
  • Population sequencing to resolve complex samples such as viral and microbial communities.