Nanostring

The CGR provides open access to researchers wishing to use the NanoString® nCounter® Analysis System to analyse nucleic acids (DNA and RNA) and proteins based on novel digital colour coded barcode technology.

The NanoString® nCounter® Analysis System is an automated platform based on fluorescently labelled reporter probes which can detect single molecule and count hundreds of molecules in a single reaction. Without the biases associated with PCR amplification the system can be use also with poor quality RNA samples and it is perfectly suited for critical samples such as FFPE (formalin-fixed, paraffin-embedded) samples. The system has the ability to use low amount of input material, as low as single cells, or analyse RNA directly from lysate or from FFPE samples. Nanostring analysis provides a very high dynamic range of quantification and sensitivity.

APPLICATIONS:

• Quantification of multiple genes from a limited amount of material
• Perfect tool for validation of NGS and microarray data
• Pathway and expression analyses of defined gene sets
• Biomarker validation
• Multiplex up to 800 targets in a single reaction making a very price competitive alternative to qPCR

If this is the first time using the service please contact the facility in advance using the enquiry form regarding your project ideas. We can answer any questions that you may have, and advise you on what technique would suit your needs. You will take care to order the necessary reagents directly from Nanostring.