The Chromium controller from 10x Genomics is an automated, tabletop library preparation platform leveraging advanced microfluidics and single-molecule barcoding technologies for multi-dimensional single-cell analysis.
The Chromium is most commonly used to investigate the gene expression of single cells within a population enabling interrogation of heterogeneous cell populations and clustering of cells into distinct types before analysis of gene expression within each cluster. However, the Chromium also offers researchers the possibility to profile the different B and T cell receptors within a sample, assay chromatin accessibility within single cells (ATAC sequencing), identify CRISPR perturbations, and antibody-based features barcoding technology can be used to quantify the levels of cell surface proteins. Furthermore, data from each of these approaches can be integrated with the single-cell gene expression data to provide even greater insights. Experiments can be conducted on up to 10,000 cells per sample and 8 samples can be run simultaneously.
Additional information about the Chromium and supported applications can be found on the 10x Genomics website.