The 10X Chromium library preparation system is an innovative system to leveraging microfluidics and single molecular barcoding.
The Chromium instrument uses molecular barcoding of high molecular weight DNA to generate long-read sequencing data using Illumina short read sequencing. The instrument can be also used to generate single cell transcriptome data and link this to cell populations.
Based on providing high molecular weight DNA, (≥50kb) the Chromium instrument allows individual barcoding of long stretches of DNA. The long DNA fragments are subsequently sheared to a smaller sizes compatible with Illumina sequencing, each retaining the barcode assigning it to the larger fragment. The libraries are then sequenced on our Illumina HiSeq platforms.
- Resolve genome sequencing for highly repetitive regions
- De Novo assembly of novel diploid genomes
- Long-range haplotyping, establishment of cis or trans relationships between variants
- Generation of long range phase blocks
- Detection of structural variants
- Exome Capture
The Chromium instrument can be used to allow for the RNA expression profiling of single cells within population. RNA transcripts from individual cells are barcoded to allow full transcriptome sequencing of individual cells. From 1000 to 10,000 cells per experiment, can be profiled to generate a single library for sequencing. Using the transcription profile, you can identify individual cell types in the population.
- Determination of cell cycle phase
- Differential expression in cell populations
- Interrogation of heterogeneous cell populations
Additional details about the technology and applications can be found on the 10x Genomics website.