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Sequencing

Genomic data are at the heart of a multitude of biological and medical research areas.

The development of NGS technology enabled genome-scale sequencing projects to be undertaken at a fraction of the time and cost of previous technologies. This sequencing revolution has unlocked the potential for new lines of research whilst revitalising existing projects. NGS technologies still represent a substantial investment to individual labs and require a great deal of technical and bioinformatic expertise. As a dedicated Centre offering all major platforms, the CGR enables researchers to access the very latest technologies as well as established platforms. We facilitate best practices to ensure individual clients receive the maximum return on their investment. At the CGR we provide access to all major sequencing and array platforms, offering a wide range of applications, with complementary advice, informatics and analytical processing of data. The CGR’s status as a reference site often facilitates access to the latest technology prior to general release. ‌

The CGR offers access to technologies from:

  • Illumina
  • Pacific Biosciences
  • 10x Genomics
  • Agilent
  • NimbleGen
  • Affymetrix

The CGR provides sequence capture techniques to enrich specific genomic regions prior to high-throughput sequencing.

Such enrichments enable the capture of regions corresponding to complete genome loci such as capture of whole exomes. This facilitates analysis of variants (e.g. SNPs and INDELs) in a statistically relevant number of samples.

The CGR is a certified service provider for both the Agilent SureSelect and NimbleGen capture platforms.

Moreover CGR has extensive experience in microarrays. We have consequently developed state-of-the-art array facilities, services and informatics systems to address the requirements of current research. Arrays are an effective and efficient way of conducting expression profiling assays for quantification of expression levels of known transcripts.

Due to the rapidly expanding demand for NGS and the evolution of higher throughput sequencing technologies, CGR has also invested in automation.

CGR has implemented automated protocols using a range of liquid handling robotic systems from Tecan, TTP Labtech, Formulatrix Mantis and Beckman Coulter for library construction and enrichment on all sequencing platforms, enabling efficient, cost-saving assay miniaturization and improved precision and accuracy.

The CGR portfolio of platforms and technologies enable solutions to be tailored according to individual project requirements. As each project has its own goals and requirements, we can guide you to choose the most suitable platform and protocol for your individual needs. Will we discuss these options with you at the outset and provide you with the latest sample requirements and technical information.

The CGR is a certified service provider for Illumina and PacBio.

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