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Engineering Nitric Oxide Delivery Platforms for the Treatment of Multidrug-Resistant Infections

Funding
Self-funded
Study mode
Full-time
Start date
Subject area
Engineering

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Overview

This project develops nitric-oxide-releasing polymer platforms to tackle drug-resistant infections. The student will design electrospun and 3D-printed materials that deliver controlled NO doses directly to infection sites and test their antimicrobial performance against clinically relevant pathogens. The work combines biomaterials engineering, polymer chemistry, and microbiology to create next-generation non-antibiotic therapies.

About this opportunity

Antimicrobial resistance (AMR) causes more than 1.2 million deaths each year and places severe pressure on healthcare systems. Without new treatments, AMR could lead to 10 million deaths annually and a global economic burden of 100 trillion dollars by 2050. These figures highlight the urgent need for alternatives to conventional antibiotics, which are increasingly ineffective as bacteria rapidly evolve resistance.

This challenge is intensified by the prevalence of biofilms, which occur in over 80 percent of bacterial infections. Biofilms are structured microbial communities surrounded by a protective matrix that shields bacteria from the immune system and severely limits antibiotic penetration. Within these environments, bacteria adopt altered metabolic states and show dramatically increased tolerance to antimicrobials. Biofilm-associated bacteria can require up to 1,000 times higher antibiotic doses for inhibition, rendering many standard therapies ineffective. Together, multidrug-resistant (MDR) pathogens and persistent biofilms create a major barrier to current antimicrobial strategies and highlight the need for innovative therapies with new mechanisms of action.

Nitric oxide (NO) offers a promising solution. It has broad-spectrum antibacterial and antibiofilm activity and acts through mechanisms fundamentally different from traditional antibiotics, making it a strong candidate for treating MDR infections. Our laboratory has already developed several NO-releasing biomaterial platforms, providing a strong foundation for the next stage of development.

This PhD project will advance the therapeutic use of NO by creating polymer-based delivery platforms engineered to release controlled levels of NO directly at sites of infection. Working at the interface of polymer chemistry, biomaterials engineering, and microbiology, the student will design and fabricate electrospun and 3D-printed NO-releasing scaffolds optimised for antimicrobial performance.

The project will provide hands-on experience in synthesising NO-donor functional polymers and processing them into nanofibrous and architected 3D structures. A broad suite of characterisation methods, including spectroscopy, rheology, thermal analysis, and mechanical testing, will be used to study material properties and release behaviour. Antibacterial and antibiofilm activity will be assessed using in vitro assays with clinically relevant Gram-positive and Gram-negative pathogens. As the project progresses, more advanced biological models may be used to explore therapeutic potential under physiologically relevant conditions.

This studentship offers a dynamic, interdisciplinary research environment and the opportunity to contribute to innovative, non-antibiotic antimicrobial technologies with real translational potential. The skills gained will span materials science, nanotechnology, drug delivery, and infection biology, providing an excellent foundation for future careers in academia or industry.

Further reading

  1. Antimicrobial nitric oxide releasing gelatin nanomparticles to combat drug resistant bacterial or fungal infections. E Myles, RA D’Sa, J Aveyard. Nanoscale advancess 2025, 7, 3096-3113
  2. 3D printable gelatin/nisin biomaterial inks for antimicrobial tissue engineering applications. M Dallos Ortega, J Aveyard, A Ciupa, RJ Poole, D Whetnall, JG Behnsen, RA D’Sa Mater Adv 2024, 5, 7729-7746
  3. Nitric oxide releasing coatings for the prevention of viral and bacterial infections J Aveyard, S Richards, M Li, G Pitt, GL Hughes, A Akpan, R Akhtar, A Kazaili, RA D’Sa Biomater. Sci., 2024, 12, 4664-4681
  4. Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications M Li,  J Aveyard, KG Doherty, RC Deller, RL Williams, KN Kolegraff, SB Kaye, RA D’Sa ACS Mater. Au 2022, 2, 2, 190–203
  5. Antimicrobial nitric oxide releasing contact lens gels for the treatment of microbial keratitis JL Aveyard, RC Deller, R Lace, RL Williams, SB Kaye, KN Kolegraff, J Curran and RA D’Sa ACS Mater and Interf. 2019, 11, 41, 37491-37501

 

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Who is this for?

Candidates will have, or be due to obtain, a Master’s Degree or equivalent in a relevant subject. Exceptional candidates with a First Class Bachelor’s Degree in an appropriate field or significant relevant experience will also be considered.

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How to apply

  1. 1. Contact supervisors

    Candidates wishing to apply should complete the University of Liverpool application form to apply for a PhD in Materials Engineering

    Please review our guide on How to apply for a PhD | Postgraduate research | University of Liverpool carefully and complete the online postgraduate research application form to apply for this PhD project.

    Please ensure you include the project title when applying.

    Supervisors Email address
    Prof Raechelle D’Sa r.dsa@liverpool.ac.uk
    Dr. Jenny Hanson Zippy78@liverpool.ac.uk
  2. 2. Prepare your application documents

    You may need the following documents to complete your online application:

    • A research proposal (this should cover the research you’d like to undertake)
    • University transcripts and degree certificates to date
    • Passport details (international applicants only)
    • English language certificates (international applicants only)
    • A personal statement
    • A curriculum vitae (CV)
    • Contact details for two proposed supervisors
    • Names and contact details of two referees.
  3. 3. Apply

    Finally, register and apply online. You'll receive an email acknowledgment once you've submitted your application. We'll be in touch with further details about what happens next.

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Fees and funding

Your tuition fees, funding your studies, and other costs to consider.

Tuition fees

If you’re a UK, Channel Islands, Isle of Man or Republic of Ireland student, tuition fees for academic year 2025/26 are:

  • Full-time: £5,006 per year
  • Part-time: £2,503 per year.

If you’re an international student, tuition fees for academic year 2026/27 are:

  • Full-time: £25,350 or £32,200 per year
  • Part-time: £12,650 or £16,100 per year.

Please note, the tuition fee you pay will reflect the nature of your research project. Some research projects incur a higher fee than others, for example if you’re required to undertake laboratory work. You’ll be informed of the fee for your intended PhD in your offer letter.

The UK fees stated are for academic year 2025/26 and may be subject to change for academic year 2026/27. The international fees stated are for academic year 2026/27 and may be subject to change for academic year 2027/28. The University may administer inflationary rises to international fees in subsequent academic years as you progress through the course.


Additional costs

We understand that budgeting for your time at university is important, and we want to make sure you understand any costs that are not covered by your tuition fee. This could include buying a laptop, books, or stationery.

Find out more about the additional study costs that may apply to this project, as well as general student living costs.


Funding your PhD

If you're a UK national, or have settled status in the UK, you may be eligible to apply for a Postgraduate Doctoral Loan worth up to £30,301 to help with course fees and living costs.

There’s also a variety of alternative sources of funding. These include funded research opportunities and financial support from UK research councils, charities and trusts. Your supervisor may be able to help you secure funding.


We've set the country or region your qualifications are from as United Kingdom.

Scholarships and bursaries

We offer a range of scholarships and bursaries that could help pay your tuition fees and living expenses.

Duncan Norman Research Scholarship

If you’re awarded this prestigious scholarship, you’ll receive significant funding to support your postgraduate research. This includes full payment of your PhD fees and a cash bursary of £23,000 per year while you study. One award is available in each academic year.

John Lennon Memorial Scholarship

If you’re a UK student, either born in or with strong family connections to Merseyside, you could be eligible to apply for financial support worth up to £12,000 per year for up to three years of full-time postgraduate research (or up to five years part-time pro-rata).

Sport Liverpool Performance Programme

Apply to receive tailored training support to enhance your sporting performance. Our athlete support package includes a range of benefits, from bespoke strength and conditioning training to physiotherapy sessions and one-to-one nutritional advice.

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Contact us

Have a question about this research opportunity or studying a PhD with us? Please get in touch with us, using the contact details below, and we’ll be happy to assist you.

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