Health & Wellbeing
51
11.2
Proteomics
Keywords
Proteomics, protein chemistry, protein mass
spectrometry, protein quantification, protein science
Expertise
Proteomics analyses are offered by the Proteomics and
Biological Mass Spectrometry Science Research
Facility. Our primary technology is mass spectrometry,
and our instrumentation suite comprises a
comprehensive range of instrumentation and facilities,
ranging from ion traps to more sophisticated Orbitrap
and QToF/ion mobility instruments. We operate almost
exclusively in nanoflow mode, delivering samples at
300
nL/minute. Surrounding this instrumentation is a
range of tools for separation science and analytical
protein chemistry. We are competent in label-free and
label-mediated quantification, and have proprietary
intellectual property for multiplexed quantification.
We cater for both identification proteomics (the
identification of protein(s) in a sample) and quantitative
proteomics, where scientists want to know the absolute
or relative quantities of specific proteins in their sample.
The Proteomics and Biological Mass Spectrometry
Science Research Facility can assist with the design,
implementation and analysis of proteomics experiments,
including protein biomarker development and
quantification and the drug characterisation of
recombinant and therapeutic proteins.
Capabilities and facilities
•
Chromatographic (soft gel, FPLC, BioLC systems)
and gel-based protein separations
•
Micromass Q-TOFmicro mass spectrometer/
nanoAcquity: intact protein mass verification
•
ThermoElectron LTQ mass spectrometer/Dionex
3000
nanoLC for metabolite analysis
•
ThermoElectron Polaris-Trace GC/MS system for
volatile analysis
•
Waters GC-TOF Premier GS/MS system for volatile
analysis
•
Waters Synapt HDMS/nanoAcquity for proteomics
discovery and quantification, and protein
characterisation
•
Waters Xevo QqQ/nanoAcquity for high specificity
and selectivity targeted quantification
•
Thermo Velos Orbitrap/nanoAcquity for ultra high
resolution discovery and quantification MS
•
Thermo Fisher QExactiv Orbitrap/Dionex nanoLC
for ultra high resolution discovery and
quantification MS
•
Synapt G2/nanoAquity high resolution ion mobility
QToF for proteomics discovery and quantification,
de novo sequencing and protein characterisation
•
Bruker Amazon high speed ion trap with ETD/
nanoAcquity for proteomics discovery and
quantification, and protein characterisation
•
Bruker Ultraflex Extreme 1kHz MALDI-TOF/TOF for
peptide mass fingerprinting, de novo sequencing
and MS imaging.
All instrumentation is supported by a dedicated IT
network, linking instruments to file servers, local search
engines and network attached storage (60Tbytes
RAID5 storage).
Relevant centres and groups
•
Proteomics and Biological Mass Spectrometry
Science Research Facility
•
Technology Directorate.
