Molecular Biology and Molecular Mechanisms of Disease


Selected Publications


Supharattanasitthi W, Carlsson E, Sharif U, Paraoan L. CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection.

Sci Rep 2019 9:174. doi: 10.1038/s41598-018-36740-2.

View abstract on Pubmed link:


Stem Cells

Pluripotent stem cells following first gene editing of AMD risk factor (Sci Rep 2019 9:174)

Porter LF, Saptarshi N, Fang Y, Rathi S, den Hollander AI, de Jong EK, Clark SJ, Bishop PN, Olsen TW, Liloglou T, Chavali VRM, Paraoan L. Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genes.

Clin Epigenetics. 2019 11(1):6. doi: 10.1186/s13148-019-0608-2.

View abstract on Pubmed link:

Sharif U, Mahmud NM, Kay P, Yang YC, Harding SP, Grierson I, Kamalden TA, Jackson MJ, Paraoan L. Advanced glycation end products-related modulation of cathepsin L and NF-κB signalling effectors in retinal pigment epithelium lead to augmented response to TNFα.

Epub 2018 Oct 19. J Cell Mol Med. 2019 23(1):405-416. doi: 10.1111/jcmm.13944. Epub 2018 Oct 19.

View abstract on Pubmed link:

Butler JM, Hall N, Narendran N, Yang YC, Paraoan L. Identification of candidate protective variants for common diseases and evaluation of their protective potential.

BMC Genomics. 2017 18(1):575. doi: 10.1186/s12864-017-3964-3. 

View abstract on Pubmed 

Awais, Spiller, White, Paraoan. p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma. Br J Cancer. 2016 Sep 1. PMID: 27584665. View Abstract on PubMed

Sant'Anna R, Navarro S, Ventura S, Paraoan L, Foguel D. Amyloid properties of the leader peptide of variant B cystatin C: implications for Alzheimer and Macular Degeneration. FEBS Lett. 2016 Feb 10. PMID: 26865059 View abstract on PubMed


Butler JM, Sharif U, Ali M, McKibbin M, Thompson JP, Gale R, Yang YC, Inglehearn C, Paraoan L. A missense variant in CST3 exerts a recessive effect on susceptibility to age-related macular degeneration resembling its association with Alzheimer's disease. Hum Genet. 2015 Apr 19. PMID: 25893795 View abstract on Pubmed


Hallstrom KN, Srikanth CV, Agbor TA, Dumont CM, Peters K, Paraoan L, Casanova JE, Boll EJ, McCormick BA. PERP, a Host Tetraspanning Membrane Protein, is Required for Salmonella-Induced Inflammation. Cell Microbiol. 2014 Dec 9. PMID: 25486861 View abstract on Pubmed


Soriano-Romaní L, García-Posadas L, López-García A, Paraoan L, Diebold Y. Thrombospondin-1 induces differential response in human corneal and conjunctival epithelial cells lines under in vitro inflammatory and apoptotic conditions. Exp Eye Res. 2015 May;134:1-14. doi: 10.1016/j.exer.2015.03.004 View Abstract on Pubmed


Kay P, Yang YC, Hiscott P, Gray D, Maminishkis A, Paraoan L. Age-related changes of cystatin C expression and polarized secretion by retinal pigment epithelium: potential age-related macular degeneration links. Invest Ophthalmol Vis Sci. 2014 Feb 14. PMID: 24458156 View abstract on Pubmed


Kay P, Yang YC, Paraoan L. Directional protein secretion by the retinal pigment epithelium: roles in retinal health and the development of age-related macular degeneration. J Cell Mol Med. 2013 May 11. PMID: 23663427 View abstract on Pubmed


Davies L, Spiller D, White M R H, Grierson I, Paraoan L. PERP expression stabilizes active p53 via modulation of p53-MDM2 interaction in uveal melanoma cells. Cell Death and Disease 2011 Mar 31; 2:e136. PMID: 21451571 View abstract on Pubmed


O'Reilly S, Pollock N, Currie L, Paraoan L, Clark AF, Grierson I. Inducers of cross-linked actin networks in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2011 Sep 21;52(10):7316-24. doi: 10.1167/iovs.10-6692 View abstract on Pubmed


Paraoan L, Hiscott P, Gosden C, Grierson I. Cystatin C in macular and neuronal degenerations: Implications for mechanism(s) of Age-related Macular Degeneration. Vision Res. 2010 mar 31;50(7):737-742. PMID: 19917302 View abstract on Pubmed


Davies L, Gray D, Spiller D, White MRH, Damato B, Grierson I, Paraoan L. P53 apoptosis mediator PERP: localization, function and caspase activation in uveal melanoma. J Cell Mol Med. 2009 13;8B:1995-2007. PMID: 19040420 View abstract on Pubmed


Paraoan L, Gray D, Hiscott P, Garcia-Fianana M, Lane B, Damato B, Grierson I. Cathepsin S and its inhibitor Cystatin C: imbalance in uveal melanoma. Frontiers in Bioscience. 2009 Jan 1;14:2504-2513. PMID: 19273215 View abstract on Pubmed


Paraoan L, Gray D, Hiscott P, Ebrahimi B, Damato B, Grierson I. Expression of p53-induced apoptosis effector PERP in primary uveal melanomas: downregulation is associated with aggressive type. Exp Eye Res. 2006 Oct;83(4):911-9. PMID: 16784742 View Abstract on PubMed


Paraoan L, Grierson I. Focus on molecules: Cystatin C. Exp Eye Res. 2007 Jun;84(6):1019-20. PMID: 16626703 View Abstract on PubMed


Ratnayaka A, Paraoan L, Spiller DG, Hiscott P, Nelson G, White MR, Grierson I. A dual Golgi- and mitochondria-localised Ala25Ser precursor cystatin C: An additional tool for characterising intracellular mis-localisation leading to increased AMD susceptibility. Exp Eye Res. 2007 Jun;84(6):1135-9. PMID: 16635487 View Abstract on PubMed


Paraoan L, Grierson I, Maden BE. Fate of cystatin C lacking the leader sequence in RPE cells. Exp Eye Res. 2003 Jun;76(6):753-6. PMID: 12742358 View Abstract on PubMed


Paraoan L, Grierson I, Maden BE. Analysis of expressed sequence tags of retinal pigment epithelium: cystatin C is an abundant transcript. Int J Biochem Cell Biol. 2000 Apr;32(4):417-26. PMID: 10762067 View Abstract on PubMed


O'Reilly S, Pollock N, Currie L, Paraoan L, Clark AF, Grierson I. Inducers of cross-linked actin networks in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2011 Sep 21;52(10):7316-24. PMID: 21849423 View Abstract on PubMed


Wade NC, Grierson I, O'Reilly S, Hoare MJ, Cracknell KP, Paraoan L, Brotchie D, Clark AF. Cross-linked actin networks (CLANs) in bovine trabecular meshwork cells. Exp Eye Res. 2009;89(5):648-659. PMID: 19540832 View abstract on Pubmed


Cryan LM, Paraoan L, Hiscott P, Damato B, Grierson I, Gray D, Farrell M, Doherty GA, Fitzgerald DJ, O'Brien C. Expression of COX-2 and prognostic outcome in uveal melanoma. Curr Eye Res. 2008 Mar 3;33:117-184. PMID: 18293189 View Abstract on Pubmed


Ratnayaka A, Paraoan L, Nelson G, Spiller DG, White MR, Hiscott P. Trafficking of osteonectin by retinal pigment epithelial cells: evidence for basolateral secretion. Int J Biochem Cell Biol. 2007;39(1):85-92. PMID: 17035068 View Abstract on PubMed


Hiscott P, Paraoan L, Choudhary A, Ordonez JL, Al-Khaier A, Armstrong DJ. Thrombospondin 1, thrombospondin 2 and the eye. Prog Retin Eye Res. 2006 Jan;25(1):1-18. PMID: 15996506 View Abstract on PubMed


Ordonez JL, Paraoan L, Hiscott P, Gray D, Garcia-Finana M, Grierson I, Damato B. Differential expression of angioregulatory matricellular proteins in posterior uveal melanoma. Melanoma Res. 2005 Dec;15(6):495-502. PMID: 16314734 View Abstract on PubMed


Paraoan L, Ratnayaka A, Spiller DG, Hiscott P, White MR, Grierson I. Unexpected intracellular localization of the AMD-associated cystatin C variant. Traffic. 2004 Nov;5(11):152-65. PMID: 15479453 View Abstract on PubMed


Hiscott P, Hagan S, Heathcote L, Sheridan CM, Groenewald CP, Grierson I, Wong D, Paraoan L. Pathobiology of epiretinal and subretinal membranes: Possible roles for the matricellular proteins thrombospondin 1 and osteonectin (SPARC). Eye. 2002 Jul;16(4):393-403. PMID: 12101446 View Abstract on PubMed


Paraoan L, White MR, Spiller DG, Grierson I, Maden BE. Precursor cystatin C in cultured retinal pigment epithelial cells: evidence for processing through the secretory pathway. Mol Membr Biol. 2001 Jul-Sep;18(3):229-36. PMID: 11681790 View Abstract on PubMed